| Popis: |
Flavonoids are plant-specific antioxidant compounds that modulate plant development, which include flavonols and anthocyanins subclasses. InArabidopsis thaliana, mutants in genes encoding each step in the flavonoid biosynthetic pathway have been isolated. We used these mutants to examine the role of flavonols in initiation and emergence of lateral roots and asked whether this regulation occurs through scavenging ROS. Thett4mutants have a defect in the first committed step of flavonoid biosynthesis and have increased lateral root emergence. This phenotype was reversed by both genetic and chemical complementation. Using these flavonoid biosynthetic mutants, we eliminated roles for anthocyanins and the flavonols, quercetin and isorhamnetin, in controlling lateral root development. Thett7-2 mutant has a defect in a branchpoint enzyme blocking quercetin biosynthesis that led to elevated levels of kaempferol and reduced lateral roots. Kaempferol accumulated within lateral root primordia and was significantly increased intt7-2. Thee data are consistent with kaempferol acting as a negative regulator of lateral root emergence. We examined ROS accumulation above and within the primordia using a general ROS sensor and identified increased signal above the primordia of thett4andtt7-2mutants compared to wild type. Using a superoxide specific sensor, we detected a decrease in signal within the primordia oftt7-2, but not thett4mutant, compared to wild type. Together, these results support a model in which increased level of kaempferol intt7-2 leads to a reduction in superoxide concentration in the lateral root primordia thereby reducing ROS-stimulated lateral root emergence. |
