The influence of poly(ethylene glycol) ether tetrasuccinimidyl glutarate on the structural, physical, and biological properties of collagen fibers
| Autor: | India R. Sweeney, Dimitrios I. Zeugolis, Oded Shoseyov, Mohsen Miraftab, Zvi Shtein, Colm O'Dowd, Anna Sorushanova, Anne Maria Mullen, Sigal Meirovich, Abhay Pandit, Mohammad Sanami |
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| Rok vydání: | 2015 |
| Předmět: |
Materials science
technology industry and agriculture Biomedical Engineering Ether 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Biomaterials chemistry.chemical_compound chemistry Polymer chemistry Genipin medicine Denaturation (biochemistry) Fiber Glutaraldehyde Swelling medicine.symptom 0210 nano-technology Ethylene glycol Carbodiimide |
| Zdroj: | Journal of Biomedical Materials Research Part B: Applied Biomaterials. 104:914-922 |
| ISSN: | 1552-4973 |
| Popis: | Various chemical, natural, or synthetic in origin, crosslinking methods have been proposed over the years to stabilise collagen fibers. However, an optimal method has yet to be identified. Herein, we ventured to assess the potential of 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate, as opposed to glutaraldehyde (GTA), genipin and carbodiimide, on the structural, physical and biological properties of collagen fibers. The 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate induced an intermedium surface smoothness, denaturation temperature and swelling. The 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate fibers had significantly higher stress at break values than the carbodiimide fibers, but significantly lower than the GTA and genipin fibers. With respect to strain at break, no significant difference was observed among the crosslinking treatments. The 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate fibers exhibited significantly higher cell metabolic activity and DNA concentration that all other crosslinking treatments, promoted consistently cellular elongation along the longitudinal fiber axis and by day 7 they were completely covered by cells. Collectively, this work clearly demonstrates the potential of 4-star poly(ethylene glycol) ether tetrasuccinimidyl glutarate as collagen crosslinker. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 914-922, 2016. |
| Databáze: | OpenAIRE |
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