| Autor: |
Sebastian Triesch, Alisandra K. Denton, Jan P. Buchmann, Vanessa Reichel-Deland, Ricardo Nuno Ferreira Martins Guerreiro, Urte Schlüter, Benjamin Stich, Andreas P.M. Weber |
| Rok vydání: |
2022 |
| DOI: |
10.1101/2022.12.06.519256 |
| Popis: |
C3-C4intermediate photosynthesis has evolved multiple times convergently and independently in the Brassicaceae, although the family lacksbona fideC4species. Evolution of C3-C4intermediate photosynthesis requires a reconfiguration of gene-regulatory networks, modified spatial expression patterns of multiple genes and ultrastructural adjustments. Mechanisms underpinning the reconfiguration of these networks are mostly unknown. In this study, we use a pan-genomic association approach to identify genomic features that might confer differential gene expression towards C3-C4intermediate traits. We found a strong correlation between transposon insertions incis-regulatory regions and the C3-C4intermediacy trait. Our study revealed 222 orthogroups where presence of a TE within a gene correlates with C3-C4intermediate photosynthesis. In this set, genes involved in the photorespiratory glycine shuttle are enriched. The P-protein of the glycine decarboxylase enzyme complex is known to play a crucial role in the establishment of the photorespiratory glycine shuttle. We found independent transposon insertions in the promoter sequences of the gene encoding the P-protein and hypothesize that these insertions lead to a spatial shift in gene expression. Our findings hint at a pivotal role of TEs in the evolution of C3-C4intermediacy, especially in mediating differential gene expression. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
