The estimation of lysine by a simplified dansylation procedure

Autor:	Graham R. Best, Terence A. Smith
Rok vydání:	1981
Předmět:	Nutrition and Dietetics Chromatography Lysine complex mixtures Toluene Chloride Fluorescence spectroscopy Thin-layer chromatography Casein hydrolysate chemistry.chemical_compound chemistry medicine bacteria Agronomy and Crop Science Food Science Biotechnology medicine.drug
Zdroj:	Journal of the Science of Food and Agriculture. 32:378-384
ISSN:	1097-0010 0022-5142
DOI:	10.1002/jsfa.2740320411
Popis:	A method is described for the estimation of free lysine which depends on its reaction with excess dimethylaminonaphthalene sulphonyl (dans) chloride to form dans-5-aminovaleraldehyde. This product is extracted into toluene, separated by thin layer chromatography and estimated by fluorimetry. The mean recovery of lysine added to casein hydrolysate using this method was 99% (s.e. 4.3%).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::81fef0d067b28d788a57cc1240a18287 https://doi.org/10.1002/jsfa.2740320411 Zobrazit plný text záznamu Plný text