High-frequency generation of transgenic tobacco plants after modified leaf disk cocultivation withAgrobacterium tumefaciens

Autor:	Mark D. Burow, Caryl A. Chlan, Andreana Lisca, Norimoto Murai, Partha Sen
Rok vydání:	1990
Předmět:	Frequency generation Transgene fungi food and beverages Plant Science Genetically modified crops Agrobacterium tumefaciens Carbenicillin Biology biology.organism_classification Callus Shoot Botany medicine Molecular Biology medicine.drug
Zdroj:	Plant Molecular Biology Reporter. 8:124-139
ISSN:	1572-9818 0735-9640
DOI:	10.1007/bf02669766
Popis:	A modified protocol for theAgrobacterium tumefaciens-mediated transformation of tobacco (Nicotina tabacum L.) leaf disks was developed for greater recovery of transgenic plants. Modifications include transformation ofAgrobacterium by a freeze-thaw procedure, initial cocultivation of leaf disks andAgrobacterium under vacuum, subsequent growth with nurse cells for one week, rooting of shoots in medium lacking carbenicillin, longer, growth in rooting medium, and a shortened “hardening” step. By this procedure, an average of 1.3 kanamycin-resistant calli were obtained per leaf disk, and 38% of, the callus cultures used were regenerated to produce 133 independently transformed tobacco plants.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::81f3f2357600cb04044fce0644bc0d33 https://doi.org/10.1007/bf02669766 Zobrazit plný text záznamu Plný text ve formátu PDF