| Popis: |
Mesenchymal stem cells (MSCs), as multipotent stem cells, can be obtained from many tissues/organs including bone marrow, adipose tissue, dental pulp, and amniotic or synovial fluids1. MSCs are unique cells that can support functions of many tissues and organs by releasing soluble factors such as growth factors, anti-apoptotic and anti-inflammation factors, and they can synthesize extracellular matrix (ECM) mainly composed of collagen fibrils, a key component of tissues. In damaged tissues, MSCs can help the formation of new blood vessels by secreting angiogenic factors. Therefore, MSCs are very helpful for reducing the inflammation2, decreasing the progress of autoimmune diseases3 and preventing rejection of transplant4. Our objective in this study is to use MSCs as supportive /feeder cells for the maintenance of liver cells including cell adhesion, cell growth and proliferation other biological activity in microfluidic chip by benefitting from such protective and regenerative properties of MSCs for tissues. To accomplish this aim, as part of a liver-on-a-chip study, we investigate the behavior of MSCs in microfluidic (MF) culture by monitoring cell viability, proliferation, cell numbers, and the expression of MSC-specific genes. Moreover, we will investigate the effect of MSCs on the viability and maintenance of liver cells within the MF-chip conducted by co-culture of MSCs with liver cells. |
