INVESTIGATION OF 2548G>A LEPTINE GENE POLYMORPHIC VARIANT IMPACT ON RISK OF NON-ALCOHOLIC FATTY LIVER DISEASE IN PATIENTS WITH TYPE 2 DIABETES MELLITUS
| Autor: | Tetiana Tyzhnenko, Kateryna Misiura, Nonna Kravchun, Maryana Gorshunska, Artem Pochernyaev, Nataliya Krasova, Alexander Gladkih, Zhanna Leshchenko, Ganna Fedorova, Olga Plohotnichenko, Olena Hromakovska, Alla Kolesnikova, Eugene Jansen, Yurii Karachentsev, Viktoriia Poltorak |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Problems of Endocrine Pathology. 79:42-52 |
| ISSN: | 2518-1432 2227-4782 |
| Popis: | Background. It is known that single nucleotide polymorphisms (SNPs) in adipokine genes can influence the development of pathological conditions associated with obesity, type 2 diabetes mellitus, non-alcoholic fatty liver disease and their complications. In this study, we aimed to investigate the link between common -2548G>A (rs7799039) promoter variant of the human leptin gene (LEP) with leptin levels in type 2 diabetes patients with non-alcoholic fatty liver diseasese. Materials and methods. 61 patients with T2D aged from 28 to 80 years old (34 men/27 women, age 56.40±0.62 yrs, diabetes duration 7.72±0.45 yrs, BMI 32.20±0.43 kg/m2, WHR 1,00 ±0,01, HbA1c 7.80±0.19 %) with varying degrees of glycemic control and overweight, without renal insufficiency and 51 sex and age-match control subjects were examined. Genotyping according to SNP LEP 2548G>A was performed using the polymerase chain reaction method with appropriate primers and HhaI endonuclease. Results. In our study of T2DM patients with NAFLD compared to T2DM patients without NAFLD features of dyslipidemia i.e. significant increase in triglycerides (p A polymorphic variant of the LEP gene can be used as a basis for personalized prevention and the formation of risk groups for the development of NAFLD. |
| Databáze: | OpenAIRE |
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