| Autor: |
Stefan Dobosz, Leyland Fraser, H. Kuzminski, J. Strzezek, I. Babiak, K. Goryczko |
| Rok vydání: |
1999 |
| Předmět: |
|
| Zdroj: |
Aquaculture Research. 30:707-710 |
| ISSN: |
1355-557X |
| DOI: |
10.1046/j.1365-2109.1999.00381.x |
| Popis: |
The effects of extender composition and freezing rate on cryopreservation efficiency of refrigerated spermatozoa of rainbow trout Oncorhynchus mykiss (Walbaum) were evaluated in order to test the suitability of a computer-controlled ultrafreezer to cryopreserve milt samples obtained in field conditions and stored for several hours. A very highly significant first-order interaction between freezing rate and the type of extender was found. Six of the eight experimental variants did not differ significantly, resulting, after fertilization of eggs with cryopreserved sperm, in a range of 62.3–74.8% of eyed embryos. This procedure was effective for samples stored at 1 °C for 2 days. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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