Lignin Removal from Tobacco Stem with Laccase Improved by Synergistic Action of Weak Alkali and Tween 80
| Autor: | Guanfeng Ma, Xinping Ouyang, Lisha Zhao, Linfeng Jiang, Chen Cheng, Dongxue Jin |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Laccase Environmental Engineering Renewable Energy Sustainability and the Environment 020209 energy technology industry and agriculture food and beverages 02 engineering and technology Alkali metal complex mixtures 01 natural sciences chemistry.chemical_compound chemistry Solubilization 010608 biotechnology Enzymatic hydrolysis Specific surface area 0202 electrical engineering electronic engineering information engineering Lignin Organic chemistry Cellulose Waste Management and Disposal Dissolution |
| Zdroj: | Waste and Biomass Valorization. 10:3343-3350 |
| ISSN: | 1877-265X 1877-2641 |
| DOI: | 10.1007/s12649-018-0346-2 |
| Popis: | Lignin removal from lignocellulosic materials plays a great role in the utilization cellulose with high efficiency. In order to improve the quality of reconstituted tobacco sheet, lignin existed in tobacco stem was removed by enzymatic hydrolysis with laccase enhanced by weak alkali treatment together with the addition of Tween 80 into laccase treatment system. It is found that lignin was partially removed by laccase. The treatment of tobacco stem with NaHCO3 resulted in the dissolution of lignin, and hence increased the porosity and specific surface area of the pretreated tobacco stem. Consequently, laccase could access to the tobacco stem more completely. The surface activity and dispersion of Tween 80 improved laccase activity, solubilization of degraded products of lignin, and contact interface between laccase and tobacco stem. The synergistic action of laccase, weak alkali and Tween 80 improved the ratio of lignin removal significantly, reaching as high as 40.3%. |
| Databáze: | OpenAIRE |
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