Micropropagation of Tartary Buckwheat (Fagopyrum tataricum Gaertn.) Plants In Vitro

Autor:	Ornpapa Anugoolprasert, Wanna Mangkita, Shigeru Hisajima, Ryo Ohsawa
Rok vydání:	2007
Předmět:	Sucrose Fagopyrum tataricum biology Plant Science biology.organism_classification Acclimatization Gellan gum chemistry.chemical_compound chemistry Micropropagation Seedling Axillary bud Botany Shoot Agronomy and Crop Science
Zdroj:	Environment Control in Biology. 45:127-132
ISSN:	1883-0986 1880-554X
DOI:	10.2525/ecb.45.127
Popis:	Micropropagation of Tartary buckwheat (Fagopyrum tataricum Gaertn.) plants was examined by the culture of a reproductive organ, seeds in vitro. In initial culture, multiple shoots were induced from a single shoot derived from a 3 week old seedling. Indevidual shoots excised from multiple shoots produced multiple shoots successively at 6 week intervals when cultures were maintained in modified Murashige and Skoog's medium supplemented with 20 μM BAP, 30 g/l sucrose and 1 g/l Gellan Gum in vitro. In some conditions, protrusions were formed and further developed to axillary buds and shoots. One can calculate that about 258 (152,587,890,625) shoots could be propagated from one shoot per year. Plantlets were regenerated by rooting from single shoots in medium containing 1 μM IBA. After acclimatization, almost 100% of regenerated plants were well established in soil. Application of the present micropropagation procedure to multiply inter-specific hybrid plants between common and Tartary buckwheat plant is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::81cdea66aba42ffb74c79149466e9c04 https://doi.org/10.2525/ecb.45.127 Zobrazit plný text záznamu