Biotin Regulation of Pancreatic Glucokinase and Insulin in Primary Cultured Rat Islets and in Biotin- Deficient Rats1
| Autor: | Guillermo Romero-Navarro, Michael S. German, Franz M. Matschinsky, Juehu Wang, Antonio Velázquez, Cristina Fernandez-Mejia, Gabriela Cabrera-Valladares |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
endocrine system
medicine.medical_specialty geography geography.geographical_feature_category Glucokinase Pancreatic islets Insulin medicine.medical_treatment Biotin deficiency Islet medicine.disease chemistry.chemical_compound Endocrinology medicine.anatomical_structure Biotin chemistry Internal medicine medicine Glucose homeostasis Pancreas |
| Zdroj: | Endocrinology. 140:4595-4600 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo.140.10.7084 |
| Popis: | Biotin has been reported to affect glucose homeostasis; however, its role on pancreatic islets of Langerhans has not been assessed. In this report, we demonstrate that physiologic concentrations of biotin stimulate glucokinase activity in rat islets in culture. Using the branched DNA (bDNA) assay, a sensitive signal amplification technique, we detected relative increases in glucokinase mRNA levels of 41.5 6 13.% and 81.3 6 19% at 12 and 24 h respectively in islets treated with [10 26 M] biotin. Because glucokinase activity controls insulin secretion, we also investigated the effect of biotin on insulin release. Treatment with [10 26 M] biotin for 24 h increased insulin secretion. We extended our studies by analyzing the effect of biotin deficiency on pancreatic islet glucokinase expression and activity, as well as insulin secretion. Our results show that islet glucokinase activity and mRNA are reduced by 50% in the biotin deficient rat. Insulin secretion in response to glucose was also impaired in islets isolated from the deficient rat. These data show that biotin affects pancreatic islet glucokinase activity and expression and insulin secretion in cultured islets. (Endocrinology 140: 4595‐ 4600, 1999) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|