Investigation of MMP-9 rs3918242 and TIMP-2 rs8179090 polymorphisms in renal cell carcinoma tissues
| Autor: | Hani Alsaadoni, Burcu Çaykara, Alper Otunctemur, Sadrettin Pence, Halime Hanım Pençe |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
business.industry Biochemistry (medical) Clinical Biochemistry Matrix metalloproteinase medicine.disease Biochemistry 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Renal cell carcinoma 030220 oncology & carcinogenesis Cancer research Medicine business Molecular Biology |
| Zdroj: | Turkish Journal of Biochemistry. 45:429-434 |
| ISSN: | 1303-829X 0250-4685 |
| DOI: | 10.1515/tjb-2019-0048 |
| Popis: | Background The proteolytic activities of matrix metalloproteinases (MMP), cell surface enzymes degrading extracellular matrix, is inhibited by matrix metalloproteinase tissue inhibitors (TIMP). We aim to detect the effects of MMP-9 rs3918242 and TIMP-2 rs8179090 gene variations in renal cell cancer transformation. Methods One hundred tumor and 100 adjacent healthy tissues were obtained from the patients with renal cell cancer. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) was performed and the products carried out in agarose gel electrophoresis were visualized under UV light. Statistical analyses were performed using SPSS 22 and p-values of less than 0.05 were considered as statistically significant. Results MMP-9 rs3918242 T allele was higher in tumor tissues (36.5%) than adjacent tissues (17%) and odds ratio was found 2.8 in T allele (p0.05). Conclusion MMP-9 rs3918242 T allele, TT and CT genotypes can be used as biomarkers in determining of renal cell carcinoma. |
| Databáze: | OpenAIRE |
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