STUDIES ON YEAST DIFFERENTIATION USING ORGANIC ACID METABOLITES PART 1. DEVELOPMENT OF METHODOLOGY USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Autor:	M. Prazak, D. J. Bell, D. Rowell, P. N. Wilson, J. D. Blake
Rok vydání:	1991
Předmět:	chemistry.chemical_classification chemistry.chemical_compound Chromatography Column temperature chemistry Metabolite Quantitative analysis (chemistry) Retention time High-performance liquid chromatography Phosphoric acid Yeast Food Science Organic acid
Zdroj:	Journal of the Institute of Brewing. 97:297-305
ISSN:	0046-9750
DOI:	10.1002/j.2050-0416.1991.tb01072.x
Popis:	The separation of organic acids on the column Aminex HPX-87H from Bio-Rad is described. The relationship between column temperature and retention time was studied for both sulphuric and phosphoric acid eluants and an analytical scheme using 0.2% (v/v) phosphoric acid was developed. While the procedure is designed for the commonly occurring non-volatile acid metabolites it was demonstrated that conditions could be established for the analysis of fatty acids to C10. Difficulties in the qualitative and quantitative analysis of organic acids in beer are discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::81be31a00f89929d8f9e6bd8de7900b8 https://doi.org/10.1002/j.2050-0416.1991.tb01072.x Zobrazit plný text záznamu Plný text