Strategies in Using Analytical Restricted Access Media Columns for the Removal of Humic Acid Interferences in the Trace Analysis of Acidic Herbicides in Water Samples by Coupled Column Liquid Chromatography with UV Detection

Autor:	and Juan-Vicente Sancho, Félix Hernández, Elbert A. Hogendoorn, Bert Baumann, Carmen Hidalgo, and Ellen Dijkman
Rok vydání:	1999
Předmět:	chemistry.chemical_classification Chromatography Chemistry Analytical chemistry Restricted access Humic acid Column switching Trace analysis Solid phase extraction Reversed-phase chromatography Uv detection Column (database) Analytical Chemistry
Zdroj:	Analytical Chemistry. 71:1111-1118
ISSN:	1520-6882 0003-2700
Popis:	In this comprehensive study, analytical restricted access media (RAM) columns were investigated to determine their performance in decreasing the humic acid interference encountered in the trace analysis of acidic herbicides in environmental water samples when employing reversed-phase liquid chromatography with UV detection (RPLC-UV). The selected analytical column RAM materials are 5-μm Semi-Permeable-Surface (SPS, Regis), 5-μm Internal Surface Reversed Phase (ISRP, Pinkerton, Regis), and 5-μm Hisep (Supelco); a precolumn containing 25-μm alkyl-diol-silica (ADS) RAM material (Merck) was also included in this study. Different LC configurations were investigated, including the single RAM column mode and column switching (LC/LC), employing one RAM column in combination with an analytical C18 column, or two RAM columns. Concerning methodology, both the single-residue method (SRM) approach and the multiresidue method (MRM) approaches were tested by analyzing SPE C18 extracts of reference water samples spiked w...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::81b520ea5330c11c606eb121c0182dcb https://doi.org/10.1021/ac980918x Zobrazit plný text záznamu