Preparative isolation and gas chromatography-mass spectrometry analysis of triterpenoids in kansui radix
| Autor: | S.-F. Teng, Y.-R. Ku, Kuo-Ching Wen, Yi-Chun Lin, Jen-Wen Lin, Chang-Hui Liao |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Pharmacology
0303 health sciences Euphorbia Chromatography biology 030309 nutrition & dietetics Chemistry Plant composition 010401 analytical chemistry Euphorbiaceae biology.organism_classification Mass spectrometry 01 natural sciences 0104 chemical sciences 03 medical and health sciences Tirucallol Triterpenoid Radix Gas chromatography–mass spectrometry Food Science |
| Zdroj: | Journal of Food and Drug Analysis. 8 |
| ISSN: | 2224-6614 |
| Popis: | Two triterpenoids, euphol and tirucallol were isolated from Kansui Radix ( Euphorbia kansuiLiou, Euphorbiaceae) by a modified isolation method. These constituents were used as marker substances for quality control of Kansui Radix using a home-developed gas chromatography-mass spectrometry (GC-MS) method. The linear calibration ranges were 50.0-400.0 µg/mL (r=0.9988) for euphol and 20.0-200.0 µg/mL (r=0.9976) for tirucallol. The relative standard deviations of two marker substances for intraday and interday analyses were 0.90-3.07% and 3.93-8.17%, respectiv ely. Recoveries were euphol 98.3±1.2%, and tirucallol 84.8±.0%. The contents of two marker substances in seven crude drugs of Kansui Radix were euphol 0.10-0.19%, and tirucallol 0.05-0.07%. |
| Databáze: | OpenAIRE |
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