Specimen preparation optimization for size and morphology characterization of nanocellulose by TEM
| Autor: | Maria do Carmo Gonçalves, Rodrigo Villares Portugal, Alexandre Cassago, Liliane Cristina Battirola, Laura C. E. da Silva |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Polymers and Plastics Polymer nanocomposite Uranyl acetate Nanoparticle 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Nanocellulose Nanomaterials chemistry.chemical_compound Biological specimen chemistry Chemical engineering Transmission electron microscopy Cellulose 0210 nano-technology |
| Zdroj: | Cellulose. 27:5435-5444 |
| ISSN: | 1572-882X 0969-0239 |
| DOI: | 10.1007/s10570-020-03116-7 |
| Popis: | Nanoparticle morphology, size and dispersion are key parameters for the application of cellulose nanomaterials in various areas, such as polymer nanocomposites, catalysts, gel and so on. Transmission electron microscopy (TEM) is the most suitable technique for the morphological characterization of these particles. However, nanocellulose low contrast in TEM images is the major drawback for their adequate morphological characterization and size determination. Even though it is widespread knowledge that negative staining using uranyl acetate is the best approach for intensifying cellulose contrast, up to now few have succeeded in achieving high quality images and reliable size measurements of these nanomaterials. This protocol presents an optimization of the standard uranyl acetate protocol commonly used for biological specimens in order to suit cellulose nanomaterials. Drying method and grid conditions were proven to be the most significant variables for effective TEM specimen preparation. These guidelines could also be successfully applied to enhance the cellulose nanomaterial contrast in polymer matrices. |
| Databáze: | OpenAIRE |
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