Inhibition of ERCC5 Attenuates Proliferation and Invasion while Promotes Apoptosis in Ovarian Cancer
| Autor: | Xinhua Bu, Donglan Yuan, Yinling Zhao, Shaojun Wu, Li Jiang, Hua Qian |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
endocrine system diseases
medicine.diagnostic_test Chemistry Wnt signaling pathway RNA medicine.disease 030226 pharmacology & pharmacy female genital diseases and pregnancy complications 03 medical and health sciences 0302 clinical medicine Western blot Apoptosis Cell culture Skov3 cell medicine Ovarian cancer cells Cancer research Ovarian cancer |
| Zdroj: | Indian Journal of Pharmaceutical Sciences. 82 |
| Popis: | The present investigation dealt with differential expression of ERCC5 in different ovarian cancer cells and the potential mechanism involved. By detecting the expression in both SKOV3 and Caov4 cell lines, it was found that the expression of ERCC5 in SKOV3 was significantly higher than that in Caov4. An SKOV3 cell line with low ERCC5 expression by ribonucleic acid interference and Caov4 cell line with high ERCC5 expression by transient transfection was constructed to compare the effect of ERCC5 abnormal expression on the biological behaviour of ovarian cancer cells. The results showed that high expression of ERCC5 could significantly promote proliferation and invasion of ovarian cancer but inhibit apoptosis, while low expression of ERCC5 could significantly promote apoptosis and inhibit proliferation and invasion. The effect of ERCC5 abnormal expression on potential regulation pathways was studied using Western blot. It was found that ERCC5 abnormal expression could regulate the Wnt pathway. It was demonstrated that inhibition of ERCC5 expression could attenuate proliferation and invasion while promote apoptosis of ovarian cancer cells by suppressing the Wnt pathway |
| Databáze: | OpenAIRE |
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