Stimulation of the Na+/H+ exchanger in human endothelial cells activated by granulocyte- and granulocyte-macrophage-colony-stimulating factor
| Autor: | Dario Ghigo, Daniela Alessi, Gianpiero Pescarmona, Alberto Mantovani, Amalia Bosia, Jm Wang, Federico Bussolino, Francesco Michelangelo Turrini, Costanzo Costamagna |
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| Rok vydání: | 1989 |
| Předmět: |
Chemistry
medicine.medical_treatment Intracellular pH Fluorescence spectrometry Cell Biology Colony-stimulating factor Biochemistry Molecular biology Sodium–hydrogen antiporter Cytokine Granulocyte macrophage colony-stimulating factor medicine Molecular Biology Ion transporter Intracellular medicine.drug |
| Zdroj: | Journal of Biological Chemistry. 264:18284-18287 |
| ISSN: | 0021-9258 |
| DOI: | 10.1016/s0021-9258(18)51460-5 |
| Popis: | It has been shown that human endothelial cells (HEC) are stimulated to migrate and proliferate by granulocyte (G)- and granulocyte-macrophage (GM)-colony-stimulating factor (CSF) (Bussolino, F., Wang, J. M., Defilipii, P. Turrini, F., Sanavio, F., Edgell, C.-J. S., Aglietta, M., Arese, P., and Mantovani, A. (1989) Nature 337, 471-473). The rapid intracellular events initiated by these cytokines on binding to their receptors on HEC are not defined. Addition of G- or GM-CSF to HEC produced a rapid activation of Na+/H+ exchanger resulting in an increase in intracellular pH (pHi). Both cytokines induced an alkaline displacement in the pHi dependence of the exchanger without affecting the affinity for external Na+ (Nao) and the rate of exchanger. Ethylisopropylamiloride, a selective inhibitor of the Na+/H+ exchanger, inhibited the intracellular alkalinization, the migration, and proliferation induced by G- and GM-CSF. The data indicate that G- and GM-CSF initiate a rapid exchange of Na+ and H+ by means of the Na+/H+ exchanger and that this ethylisopropylamiloride-sensitive ions flux is important to the biological effects of these cytokines on HEC. |
| Databáze: | OpenAIRE |
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