| Autor: |
Veronika Csillag, Marianne Hiriart Bizzozzero, Joyce Noble, Björn Reinius, János Fuzik |
| Rok vydání: |
2022 |
| DOI: |
10.1101/2022.11.09.515875 |
| Popis: |
Understanding the routing of neuronal information requires the functional characterization of connections. Neuronal projections recruit large postsynaptic ensembles with distinct postsynaptic response types (PRTs). PRT is probed by low-throughput whole-cell electrophysiology and is not a selection criterion for single-cell RNA-sequencing (scRNA-seq). To overcome these limitations and target neurons based on specific PRTs for soma harvesting and subsequent scRNA-seq we created Voltage-Seq. To test our methodology, we established all-optical voltage imaging and recorded the PRT of 8347 periaqueductal gray (PAG) neurons evoked by the optogenetic activation of ventromedial hypothalamic (VMH) terminals. PRTs were classified and spatially resolved in the entire VMH-PAG connectome. We built an on-site analysis named VoltView to navigate soma harvesting towards target PRTs guided by a classifier which used the VMH-PAG connectome database as a reference. We demonstrated the agility of Voltage-Seq in locating VMH-driven GABAergic neurons in the PAG, solely guided by the on-site classification in VoltView. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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