Use of Percolation for the Extraction of Vasopressin1

Autor:	Brahm Muhlstock, Murray Saffran, Seymour Mishkin, Brigitte U. Caplan
Rok vydání:	1962
Předmět:	chemistry.chemical_classification Vasopressin Ethanol Chromatography Arginine Amino acid chemistry.chemical_compound Acetic acid Hydrolysis Endocrinology medicine.anatomical_structure chemistry Posterior pituitary medicine Acid hydrolysis hormones hormone substitutes and hormone antagonists
Zdroj:	Endocrinology. 70:43-46
ISSN:	1945-7170 0013-7227
DOI:	10.1210/endo-70-1-43
Popis:	Beef posterior pituitary powder was percolated with ethanol containing increasing concentrations of water and of acetic acid. The percolate with 70% ethanol containing 0.05 M acetic acid was rich in vasopressin. Further purification of vasopressin was effected by electrophoresis in 0.5 M acetic acid at 1000 volts. The best preparation of vasopressin, produced by a combination of percolation and electrophoresis, had a potency of 450 units per mg. Acid hydrolysis of this preparation yielded only the amino acids of arginine vasopressin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8167d00a2928ec6eadc8ee115369b3d6 https://doi.org/10.1210/endo-70-1-43 Zobrazit plný text záznamu