Rapid determination of the anti-cancer drug Melphalan (AlkeranTM) in human serum and plasma by automated solid phase extraction and liquid chromatography tandem mass spectrometry

Autor:	R. C. Causon, J. P. Allanson, I. D. Davies
Rok vydání:	2000
Předmět:	Analyte Chromatography Chemistry Organic Chemistry Clinical Biochemistry Selected reaction monitoring Reversed-phase chromatography Biochemistry High-performance liquid chromatography Analytical Chemistry Blood serum Liquid chromatography–mass spectrometry Sample preparation Solid phase extraction
Zdroj:	Chromatographia. 52:S92-S97
ISSN:	1612-1112 0009-5893
DOI:	10.1007/bf02493133
Popis:	A bioanalytical method for the determination of the anticancer drug Melphalan (AlkeranTM) in human serum and plasma is described. Automated solid phase extraction of the analyte is carried out with C18 sorbent packed in a 96 well format microtitre plate using a robotic sample processor. The extracts are analysed by isocratic reversed phase liquid chromatography using pneumatically and thermally assisted electrospray ionisation (TurbolonSprayTM) with selected reaction monitoring. The method is specific and sensitive, with a range of 2–400 ng mL−1 in human serum and plasma for Melphalan (sample volume 200 μl). The method is accurate and precise with intra-assay and inter-assay precision (%CV) of
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::81675fd20394d904be2a64d878d085aa https://doi.org/10.1007/bf02493133 Zobrazit plný text záznamu Full text from SpringerLink