Antimicrobial activity, cytotoxic effect and characterization of marine bivalve extracts Cerastoderma glaucum
Autor: | Mohamed H. Mona, Karolin K. Abd El-Aziz, Salwa A. El-Saidy, Gihan M. El-Khodary, Nahla E. Omran |
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Rok vydání: | 2020 |
Předmět: |
Gel electrophoresis
0303 health sciences biology Chemistry Klebsiella oxytoca Antimicrobial biology.organism_classification 01 natural sciences Yeast Microbiology Pseudomonas stutzeri 03 medical and health sciences Shigella flexneri 030301 anatomy & morphology Globicatella sulfidifaciens 0103 physical sciences General Earth and Planetary Sciences General Agricultural and Biological Sciences 010303 astronomy & astrophysics Cerastoderma glaucum General Environmental Science |
Zdroj: | Rendiconti Lincei. Scienze Fisiche e Naturali. 32:149-161 |
ISSN: | 1720-0776 2037-4631 |
Popis: | Cerastoderma glaucum, a marine bivalve inhabiting Lake Timsah, is surrounded by different pathogenic organisms. The present study evaluates the antimicrobial activities, cytotoxicity and characterization of C. glaucum extracts. Chloroform, methanol and acidic tissue extracts were prepared from C. glaucum collected during winter and summer seasons. Winter acidic extract exhibits potent antimicrobial activities against 21 bacterial, 2 yeast and 2 viral strains. The inhibition zone of this extract ranges from 10 mm against Klebsiella oxytoca, Pseudomonas stutzeri, Globicatella sulfidifaciens and Bacillus (B. badius, B. amyloliquefaciens and B. pumilus) to 24 mm against Shigella flexneri. Also, the inhibition viral activities of this extract at a concentration of 62.5 µg ml−1 against Hepatitis A virus and Herpes simplex virus type 1 (HSV-1) are 62.383% and 57.035%, respectively, with low cytotoxicity of 24.030%. Furthermore, winter acidic extract of C. glaucum has the highest total protein contents (9.8 mg ml−1) compared with the other extracts. Moreover, the sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) indicates the presence of four clear low molecular weight peptides bands; 8.588, 7.237, 4.423 and 2.692 kDa. Fourier transform-infrared (FT-IR) analysis indicates the presence of 12 functional groups of proteins in winter acidic extract of C. glaucum at appropriate wavelengths. |
Databáze: | OpenAIRE |
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