In Vivo Determination of Reduced Thiols in Rat Cerebellum Paraflocculus Following Salicylate-Induced Tinnitus by Fluorescence
| Autor: | Liu Zhao, Zhao Li, Chenxiao Zhang, Lingzhi Zhao |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Cerebellum
Microdialysis Antioxidant Homocysteine medicine.medical_treatment 010401 analytical chemistry Biochemistry (medical) Clinical Biochemistry Endogeny Glutathione 010402 general chemistry 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry chemistry.chemical_compound medicine.anatomical_structure chemistry In vivo Electrochemistry medicine Spectroscopy Cysteine |
| Zdroj: | Analytical Letters. 50:530-543 |
| ISSN: | 1532-236X 0003-2719 |
| Popis: | The increasing number of patients suffering from tinnitus may be explained by an imbalance in the antioxidant defense system and reactive oxygen species formation, suggesting the importance of the redox homeostasis in this condition. Endogenous biothiols play important roles in maintaining redox homeostasis. Hence, to understand the role of biothiols in the pathological process of tinnitus, this study demonstrates an in vivo method for monitoring the concentrations of biothiols in the paraflocculus of the rat cerebellum during tinnitus induced by the injection of salicylate. Resorufin-based fluorescent probe 1 was used as the selective probe with in vivo microdialysis. Probe 1 was premixed with microdialysates from the paraflocculus of the rat cerebellum and transferred into a cuvette for continuous-flow fluorescence. A linear relationship between the fluorescence and the concentrations of cysteine, homocysteine, and glutathione was from 1 to 15 µM, with detection limits of 128, 148, and 183 nM, r... |
| Databáze: | OpenAIRE |
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