Production of proteinase–plasminogen activators by micromycete Tolypocladium inflatum k1
| Autor: | N. S. Egorov, V. G. Kreier, T. S. Sharkova, A. A. Osmolovskiy, N. A. Baranova, E. O. Matveeva, A. V. Kurakov |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Proteases biology Activator (genetics) Plasminogen activator activity biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Fibrin Microbiology Serine 03 medical and health sciences 030104 developmental biology Tolypocladium inflatum 010608 biotechnology Extracellular biology.protein Fibrinolytic enzyme |
| Zdroj: | Applied Biochemistry and Microbiology. 52:31-35 |
| ISSN: | 1608-3024 0003-6838 |
| DOI: | 10.1134/s0003683816010129 |
| Popis: | The cultivation conditions (cultivation duration, nitrogen sources, and initial pH of the medium) of the strain Tolypocladium inflatum k1, a producer of extracellular proteases, has been optimized to achieve maximal plasminogen activator activity. It has been shown that Tolypocladium inflatum k1 forms at least two proteinases with activator activity towards plasminogen. One of them is thiol-dependent, EDTA-sensitive serine proteinase, which activates plasminogen and does not hydrolyze fibrin, indicating its higher specificity and prospects for the development of new thrombolytic agents. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|