| Autor: |
Wenjun Chen, Pingkai Peng, Yibo Xiang, Shu Huang, Sheng Li, Cui Qin, Yuping Guan |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Journal of Biomaterials and Tissue Engineering. 12:381-385 |
| ISSN: |
2157-9083 |
| DOI: |
10.1166/jbt.2022.2908 |
| Popis: |
This study intends to assess miR-653’s expression in MSCs and OSCC and discuss molecular biological mechanism of changes of EMT in MSCs through activating miR-653 in OSCC. miR-653 expression in MSCs and OSCC was detected. si-miR-653 was transfected into MSCs followed by analysis of cell proliferation by CCK-8 and clone formation assay, cell apoptosis and cycle by FCM, and the changes of transcription factor as ZEB1 and Snail by qRT-PCR. miR-653 expression in OSCC cell was up-regulated significantly from the result of q-RT-PCR detection. The proliferation of MSCs induced by miR-653 was restrained and apoptotic rate was increased after treatment with si-miR-653 along with stagnated cycle of G1/G0 staging cell. The expression of transcription factor of EMT type as ZEB1 and Snail was elevated significantly after intervention using si-miR-653. In conclusion, the proliferation of OSCC could be induced by MSCs through activation with miR-653 which might be through regulation of EMT process. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
