The Multiplex Genotyping Method for Single-Nucleotide Polymorphisms of Genes Associated with Obesity and Body Mass Index
| Autor: | A. V. Bocharova, K. V. Vagaitseva, M. M. Gavrilenko, Vadim Stepanov, V. V. Ivanov, A. A. Popovich, E. A. Trifonova |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Genetics 0303 health sciences Candidate gene Locus (genetics) Single-nucleotide polymorphism Overweight Biology medicine.disease 01 natural sciences Obesity Human genetics 03 medical and health sciences medicine 1000 Genomes Project medicine.symptom Body mass index 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Russian Journal of Genetics. 55:1282-1293 |
| ISSN: | 1608-3369 1022-7954 |
| DOI: | 10.1134/s1022795419100144 |
| Popis: | Obesity is one of the global health problems resulting in significant economic and social damage in both developed and developing countries. Overweight and obesity are key risk factors of diabetes and cardiovascular and oncological diseases that cause high morbidity and mortality. In the present paper, the method of multiplex genotyping of polymorphic variants of genes associated with obesity and variability of body mass index (BMI) was developed on the basis of multilocus PCR and MALDI-TOF mass spectrometry of DNA molecules. The frequencies of 51 single-nucleotide polymorphisms of obesity candidate genes in a population sample of Russians in Kemerovo were characterized. The results obtained were compared with the data for populations from the 1000 Genomes project. The association of markers rs12446632 of the LOC105371116 locus and rs16851483 of the RASA2 gene with BMI variability in the Russian population of Kemerovo was shown. |
| Databáze: | OpenAIRE |
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