| Autor: |
Wen Ai, Peiyi Xie, Liting Liao, Haijin Chen, Li Ling, Yulan Gao, Lei Wang, Chen Shaoyuan, Fang Yeqing, Hongcheng Fang, Yanwei Chen |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Materials Express. 12:1072-1080 |
| ISSN: |
2158-5849 |
| DOI: |
10.1166/mex.2022.2248 |
| Popis: |
As a disorder of the myocardium caused by diabetes mellitus, DCM has become a key health concern in the world. Autophagy plays an important role in the pathogenesis of DCM. Sphingosine kinase 1 benefits to cell survival and growth, and regulates the pathogenesis of many diseases, including diabetes and cardiovascular disease. Silibinin is widely used as a hepatoprotective and antioxidant agent in Asia and Europe. However, how silibinin governs DCM remains poorly understood. To this aim, the present study is to mine the role of Sphk1 in autophagy induction and cell survival in myocardial cells under high glucose treatment, and how silibinin regulates DCM. Herein, the study used the embryonic rat heart-derived myogenic H9C2 cells and adenovirus based gene manipulation was utilized. It was found that silibinin could induce autophagy in a dose-dependent manner. Overexpression of Sphk1 significantly increased mRNA expression of autophagy gene markers in H9C2 cells. Gain of function of Sphk1 significantly increased protein level of Beclin1 in H9C2 cells under treatments of low (5.5 mM) and high (25 mM) glucose. High glucose inhibited autophagy induction in H9C2 cells. Gain of function of Sphk1 compromised inhibition of on autophagy induction by high glucose. The results of the present study may assist in attaining an improved understanding of the pathogenesis of DCM, and developing novel therapies for treatment of DCM. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
