Autor: |
K. Pompe, Gerhard Heil, Hermann Heimpel, J. Krauter |
Rok vydání: |
1997 |
Předmět: |
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Zdroj: |
Acute Leukemias VI ISBN: 9783642643798 |
DOI: |
10.1007/978-3-642-60377-8_45 |
Popis: |
CD54 (ICAM-1) expression was studied on blasts of 13 cases of de novo AML and 10 cases of ALL prior to and after stimulation by interferon-gamma (IFN-γ). Furthermore, its functional activity was studied by analysis of the influence of CD54 on homologous interaction of the blasts. Prior to culture only a minority of the AML and ALL blasts displayed CD54 positivity. Timed incubation of the blasts in RPMI-1640/FCS or serum-free medium “spontaneously” increased the percentage of CD54 positive cells in 11/13 AML cases but only in 2/10 ALL cases. In AML, IFN-γ (500 IU/ml) further enhanced CD54 positivity in 6/11 cases. 2/13 cases showed no CD54 upregulation at all. In 6/10 ALL cases CD54 upregulation after IFN-γ stimulation was found. In AML ICAM-1 expression was paralleled by homologous aggregation of the blasts in that (1) in all but the two CD54 negative cases autologous cluster formation could be detected; (2) IFN-γ enhanced cluster formation in 5/6 cases in which it had enhanced CD54 upregulation; (3) incubation of the blasts in the presence of an anti-CD54 MoAb (clone 84H10) reduced the “spontaneous” and IFN-γ induced cluster formation in the majority of the cases. In ALL only 2/6 cases with CD54 upregulation showed homologous cell aggregation, which could be slightly enhanced by IFN-γ stimulation and inhibited by the anti-CD54 moAb. Taken together, CD54 expression on AML and ALL blasts is heterogeneous with respect to (1) its “constitutional” expression and (2) its “spontaneous” and IFN-γ induced upregulation. While on AML blasts CD54 seems to be funtionally active once expressed on the surface membrane, in at least some ALL cases its function seems to depend on additional activation. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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