Development and primary application of the SYBR Green I RT-qPCR assay for the detection of the transmissible gastroenteritis virus (TGEV) S gene

Autor:	Zhibiao Yang, Xiuguo Hua, Jiangping Gu, Rui Xing, Caiying Li, Congli Yuan, Xiuwei Yue, Li Cui
Rok vydání:	2011
Předmět:	chemistry.chemical_compound Plasmid Real-time polymerase chain reaction chemistry Specific primers Pcr assay SYBR Green I Porcine transmissible gastroenteritis virus Transmissible gastroenteritis virus Biology Gene Virology Molecular biology
Zdroj:	BMEI
DOI:	10.1109/bmei.2011.6098527
Popis:	This study established a SYBR Green I real-time quantitative PCR detection assay (RT-qPCR) for the detection of the porcine transmissible gastroenteritis virus (TGEV) using specific primers designed to amplify the highly conserved porcine TGEV S gene sequence. The threshold cycle (Ct) and the log plasmid copy numbers had a good linear relationship with an efficiency of 1.05 (R2 = 0.999). The advantages of utilizing this approach for the rapid detection of TGEV include excellent sensitivity, reproducibility, and low cost. Ninety-six porcine fecal samples were tested in this study, and 7 samples more than PCR assay were detected by this assay.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::81308c113f965ffec0277a83d83f4e6e https://doi.org/10.1109/bmei.2011.6098527 Zobrazit plný text záznamu