PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux

Autor:	Lucie Perry, Jiri Friml, Jan Petrášek, Ok Ran Lee, Markus Geisler, Martin Kubeš, Jozef Mravec, Milada Čovanová, Petr Skůpa, Gerald R. Fink, Eva Benková, Daniela Seifertová, Pavel Křeček, Joshua J. Blakeslee, Eva Zazímalová, Angus S. Murphy, Rodolphe Bouchard, Melinda Abas, Pankaj Dhonukshe, Christian Luschnig, Justyna Wiśniewska, Zerihun Tadele
Rok vydání:	2006
Předmět:	Auxin efflux chemistry.chemical_classification Auxin influx Intracellular auxin transport Multidisciplinary Auxin homeostasis fungi food and beverages Biology Cell biology chemistry Auxin Botany heterocyclic compounds PIN proteins Polar auxin transport Basipetal auxin transport
Zdroj:	Science. 312:914-918
ISSN:	1095-9203 0036-8075
Popis:	Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport–related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::812746272bccf5f66251c78f6f30c8af https://doi.org/10.1126/science.1123542 Zobrazit plný text záznamu Plný text ve formátu PDF