Isolation of linoleic andα-linolenic acids as COX-1 and -2 inhibitors in rose hip
| Autor: | G. Thomasen, K. N. Petersen, S. Brøgger Christensen, Anna K. Jäger |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Pharmacology
chemistry.chemical_classification Chromatography Linolenic acid Rosa canina Linoleic acid Biology Pharmacognosy biology.organism_classification High-performance liquid chromatography chemistry.chemical_compound chemistry Essential fatty acid Biochemistry Petroleum ether Polyunsaturated fatty acid |
| Zdroj: | Phytotherapy Research. 22:982-984 |
| ISSN: | 0951-418X |
| DOI: | 10.1002/ptr.2446 |
| Popis: | Rose hip has previously shown clinical efficacy in the treatment of osteoarthritis, and organic solvent extracts of rose hip have showed inhibition of cyclooxygenase-1 and -2. A petroleum ether extract of rose hip was fractioned by VLC on silica; on a C-18 column and by HPLC. Each step was COX-1/2 activity-guided. The bioassay-guided fractionation led to the isolation of linoleic acid (the IC50 for COX-1 was 85 microm and 0.6 microM for COX-2) and alpha-linolenic acid (the IC50 for COX-1 was 52 microM and 12 microM for COX-2). The COX-2/COX-1 ratio was 0.007 for linoleic acid and 0.2 for alpha-linolenic acid. Linoleic acid and alpha-linolenic acid contribute to the COX-1 and -2 inhibitory activity of rose hip. |
| Databáze: | OpenAIRE |
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