Evaluation of different culture conditions ofClostridium bifermentans DPH-1 for cost effective PCE degradation
| Autor: | Yuki Hasegawa, Takeshi Sato, Kazuhiro Takamizawa, Afroze Syeda Humayra, Young C. Chang, Izumi Nomura |
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| Rok vydání: | 2005 |
| Předmět: |
Growth medium
biology Strain (chemistry) technology industry and agriculture Biomedical Engineering Bioengineering Electron donor biology.organism_classification Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Bioremediation chemistry Yeast extract Degradation (geology) Food science Industrial and production engineering Clostridium bifermentans Biotechnology |
| Zdroj: | Biotechnology and Bioprocess Engineering. 10:40-46 |
| ISSN: | 1976-3816 1226-8372 |
| DOI: | 10.1007/bf02931181 |
| Popis: | Clostridium bifermentans strain DPH-1 has already been found to dechlorinate perchloroethylene (PCE) tocis-dichloroethylene (cis-DCE)via trichloroethylene (TCE). In this study, our investigation on different culture conditions of this DPH-1 strain was extended to find a more efficient and cost effective growth medium composition for this DPH-1 strain in bioremediation practices. Temperature dependency of strain DPH-1 showed that the growth starting time and PCE degradation at 15°C was very slow compared to that of 30°C, but complete PCE degradation occurred in both cases. For the proper utilization of strain DPH-1 in more cost effective bioremediation practices, a simpler composition of an effective media was studied. One component of the culture medium, yeast extract, had been substituted by molasses, which served as a good source of electron donor. The DPH-1 strain in the medium containing molasses, in the presence of K2HPO4 and KH2PO4, showed identical bacterial multiplication (0.135 mg protein mL−1h−1) and PCE degradation rates (0.38 μM/h) to those of the yeast extract containing medium. |
| Databáze: | OpenAIRE |
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