Validated high-performance thin-layer chromatographic method for the determination of dibenzyl cyclooctadiene lignans fromSchisandra grandiflora

Autor: B. Poornima, Katragadda Suresh Babu, Mandava Venkata Basaveswara Rao, Nissankararao Srinath, Bokka Ramesh, K. Kumar, V.U.M. Sarma, Vadaparthi P. R. Rao
Rok vydání: 2014
Předmět:
Zdroj: Journal of Planar Chromatography – Modern TLC. 27:460-465
ISSN: 1789-0993
0933-4173
DOI: 10.1556/jpc.27.2014.6.10
Popis: A simple, accurate, and rapid high-performance thin-layer chromatographic (HPTLC) method has been established and validated for the simultaneous quantification of the four biologically active dibenzyl cyclooctadiene lignans, i.e., schisandrin (1), gomisin B (2), deoxyschisandrin (3), and gomisin N (4) from the hexane extract of Schisandra grandiflora (Wall.) Hook. f. & Thoms. Separation was performed on silica gel 60 F254 plates using a saturated mixture of toluene-ethyl acetate-methanol (6:1:1 v/v) as the mobile phase. Quantitation was performed using densitometric absorption-reflection mode at 225 nm. The method was validated for its selectivity, linearity, precision, and accuracy. HPTLC was hyphenated with a mass spectrometer as an additional tool for the confirmation of the markers using an interface. The developed method is simple and convenient which can be applied for the regular quality analysis of the raw plant material used in Chinese traditional herbal formulations.
Databáze: OpenAIRE
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