Demonstration of the Usefulness of Optical Coherence Tomography in Imaging a Mouse Tail Model of Lymphedema
| Autor: | Dong Kyu Kim, Yu-Gyeong Chae, Young-Joo Sim, Ghi Chan Kim, Hui Dong Kim, Yeh-Chan Ahn, Seok Gyo Park, Ho Joong Jeong |
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| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
genetic structures medicine.diagnostic_test business.industry Magnetic resonance imaging medicine.disease eye diseases Atomic and Molecular Physics and Optics body regions Lymphedema medicine.anatomical_structure Lymphatic system Dermis Optical coherence tomography hemic and lymphatic diseases Edema Biopsy medicine sense organs Radiology medicine.symptom business Nuclear medicine Preclinical imaging |
| Zdroj: | Current Optics and Photonics. 1:132-137 |
| ISSN: | 2508-7266 |
| DOI: | 10.3807/copp.2017.1.2.132 |
| Popis: | To investigate the usefulness of optical coherence tomography (OCT) for imaging lymphedema, we directly compared it to other histological methods in a mouse model of lymphedema. We performed detailed imaging of the lymphedema lesion on a mouse tail. We imaged the mouse tail in vivo with OCT and created histopathological samples. We constructed a spectrometer-based OCT system using a fiber-optic Michelson interferometer. The light was directed to 50:50 couplers that split the light into reference and sample arms. Backscattered light from a reference mirror and the sample produced an interference fringe. An OCT image of the lymphedema model revealed an inflammatory reaction of the skin that was accompanied by edema, leading to an increase in the light attenuation in the dermal and subcutaneous layers. Similar to OCT image findings, histological biopsy showed an inflammatory response that involved edema, increased neutrophils in epidermis and subdermis, and lymphatic microvascular dilatation. Furthermore, the lymphedema model showed an increase in thickness of the dermis in both diagnostic studies. In the mouse tail model of lymphedema, OCT imaging showed very similar results to other histological examinations. OCT provides a quick and useful diagnostic imaging technique for lymphedema and is a valuable addition or complement to other noninvasive imaging tools. |
| Databáze: | OpenAIRE |
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