Fluorescent Glyconanoparticles as a Sensitive Device to Monitor Sugar-Involving Molecular Events

Autor:	Shin-Ichiro Nishimura, Reiko Sadamoto, Noriko Nagahori, Kenji Monde, Norimasa Iwasaki, Noriko Osuga, Kenichi Niikura, Akio Minami, Masamichi Shiono
Rok vydání:	2004
Předmět:	Chromatography Polymers and Plastics biology Chemistry Vesicle Nanoparticle Lectin Fluorescence Matrix (chemical analysis) Adsorption Glycolipid Photopolymer Materials Chemistry biology.protein Biophysics
Zdroj:	Polymer Journal. 36:209-218
ISSN:	1349-0540 0032-3896
DOI:	10.1295/polymj.36.209
Popis:	We prepared sugar-displayed fluorescent nanoparticles using photopolymerization of the vesicles of diacetylene-containing glycolipids. After photopolymerization, the nanoparticles exhibited fluorescent emission at 400–600 nm upon excitation at 380 nm. The binding of sugar-recognizing proteins (lectins) onto the fluorecent nanoparticles induced changes in fluorescence. The fluorescent intensity of the nanoparticles decreased with an increase in lectin concentration. On the contrary, the enzymatic release of oligosaccharides from the glyconanoparticles gave an increase in fluorescence. Using a phosphatidylcholine-type lipid as the matrix lipid of the nanoparticles, the non-specific adsorption of lectins on the surface was drastically restricted, leading to the generation of a specific response to the target proteins.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::81006af2442b048d1f450a8d3f1676a9 https://doi.org/10.1295/polymj.36.209 Zobrazit plný text záznamu