Regulatory effects of moxibustion on ubiquitin and NLRP3 proteins in colon of ulcerative colitis rats

Autor: Guang Yang, Xie-He Kong, Ling Yang, Jie Liu, Xiao-peng Ma, Jue Hong, Li-Jie Wu, Xi-ying Li, Dan Zhang, Xiao-qing Dong, Zhi Fangyuan, Yue Zhao
Rok vydání: 2020
Předmět:
Zdroj: Journal of Acupuncture and Tuina Science. 18:96-104
ISSN: 1993-0399
1672-3597
DOI: 10.1007/s11726-020-1162-3
Popis: To observe the effects of moxibustion on colonic inflammation, and the expressions of ubiquitin and nucleotide-binding oligomerization domain (Nod)-like receptor protein 3 (NLRP3) proteins in rats with ulcerative colitis (UC), and to explore the anti-inflammatory mechanism of moxibustion in the UC treatment. Clean grade male Sprague-Dawley (SD) rats were randomly divided into a normal group (NG), a model group (MG), a moxa-stick moxibustion group (MSMG) and a Western medicine group (WMG). UC model was prepared by freely drinking 35 g/L dextran sulfate sodium (DSS) solution. Bilateral Tianshu (ST 25) were selected for mild moxibustion treatment in the MSMG; mesalazine solution was intragastrically administrated in the WMG. Rats in the NG and MG were only grasped and fixed as in the MSMG without any treatment. After treatment, hematoxylin-eosin (HE) staining was performed to observe and score the colonic pathological damage under light microscope; immunofluorescence method was used to determine the expression of colonic ubiquitin protein; immunohistochemical method was used to detect the expressions of colonic interleukin (IL)-1β and NLRP3 proteins. The colon tissue was severely injured, and the pathological score was significantly increased in the MG than in the NG (P
Databáze: OpenAIRE
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