Microdetermination of plasma ATP and creatine phosphate concentrations with a luminescence biometer

Autor:	W J Ferrell, C M Jabs, H J Robb
Rok vydání:	1977
Předmět:	Chromatography biology Apyrase Biochemistry (medical) Clinical Biochemistry Firefly luciferin Phosphate Creatine Phosphocreatine chemistry.chemical_compound chemistry biology.protein Luciferase Creatine kinase Adenosine triphosphate
Zdroj:	Clinical Chemistry. 23:2254-2257
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/23.12.2254
Popis:	We optimized conditions for determination of adenosine-5'-triphosphate (ATP) and creatine phosphate from plasma extracted with ethanol/water (96/4 by vol). The procedures utilize the firefly luciferin/luciferase reaction, the bioluminescence being measured with a Du Pont Biometer. ATP is quantitated directly and creatine phosphate is quantitated by reaction with creatine kinase and ADP, after plasma ATP is removed by incubation with the enzyme apyrase. The method is applied to plasma from humans, rabbits, and rats, and possible clinical applications are discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::80fc7311f6e99e49525040f4d9c6c9c9 https://doi.org/10.1093/clinchem/23.12.2254 Zobrazit plný text záznamu