| Autor: |
Jin Boo Jeong, Gwang Hun Park, Man Hyo Lee, Hun Min Song, Jin Suk Koo, Min Ji Kwon, Mi Kyoung Kim, Jin Wook Lee, Hyun Ji Eo, Jeong Rak Lee |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Korean Journal of Plant Resources. 28:297-304 |
| ISSN: |
1226-3591 |
| DOI: |
10.7732/kjpr.2015.28.3.297 |
| Popis: |
The flower buds of Sophora japonica L (SF), as a well-known traditional Chinese medicinal herb, have been used to treat bleeding-related disorders such as hematochezia, hemorrhoidal bleeding, dysfunctional uterine bleeding, and diarrhea. However, no specific anti-cancer effect and its molecular mechanism of SF have been described. Thus, we performed in vitro study to investigate if treatment of SF affects activating transcription factor 3 (ATF3) expression and ATF3-mediated apoptosis in human colorectal cancer cells. The effects of SF on cell viability and apoptosis were measured by MTT assay and Western blot analysis against cleaved poly (ADP-ribose) polymerase (PARP). ATF3 activation induced by SF was evaluated using Western blot analysis, RT-PCR and ATF3 promoter assay. SF treatment caused decrease of cell viability and increase of apoptosis in a dose-dependent manner in HCT116 and SW480 cells. Exposure of SF activated the levels of ATF3 protein and mRNA via transcriptional regulation in HCT116 and SW480 cells. Inhibition of extracellular signal-regulated kinases (ERK) 1/2 by PD98059 and p38 by SB203580 attenuated SF-induced ATF3 expression and transcriptional activation. Ectopic ATF3 overexpression accelerated SF-induced cleavage of PARP. These findings suggest that SF-mediated apoptosis may be the result of ATF3 expression through ERK1/2 and p38-mediated transcriptional activation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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