Characterization of phytochelatin synthase and relationship with Cd-tolerance of plant

Autor:	Ryoji Nakazawa, Hiroshi Takenaga
Rok vydání:	1997
Předmět:	chemistry.chemical_classification biology ATP synthase Nicotiana tabacum Glutathione biology.organism_classification Plant cell In vitro chemistry.chemical_compound Enzyme Biosynthesis chemistry Biochemistry biology.protein Phytochelatin
Zdroj:	Plant Nutrition for Sustainable Food Production and Environment ISBN: 9789401065108
DOI:	10.1007/978-94-009-0047-9_124
Popis:	Phytochelatin (PC) synthase of tobacco cells (Nicotiana tabacum cv. BY-2) was characterized. The enzyme was most stable in a narrow pH range around 7.5. Cd2+ and glycerol stablized this enzyme in vitro. The Km for glutathione (GSH) was 8.6 mM, which was higher than the GSH concentration in the plant cell. Both of the level of induction of cellular PC in response to Cd2+ exposure and the level of Cd-tolerance were elevated by application of exogenous GSH. This suggests that cellular GSH concentration is one of the limiting factors of PC biosynthesis and Cd-tolerance.
Databáze:	OpenAIRE
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