| Autor: |
Jian Guang Zhou, Xiao Long Qian, Lan Yu, Hong Tao Wang, Le Le Wang, Qing Guo Shi, Shan Hu Li |
| Rok vydání: |
2010 |
| Předmět: |
|
| Zdroj: |
Hereditas (Beijing). 32:348-352 |
| ISSN: |
0253-9772 |
| DOI: |
10.3724/sp.j.1005.2010.00348 |
| Popis: |
PC-1(Prostate and colon gene 1) gene belongs to TPD52 (Tumor Protein D52) gene family. The expression of PC-1 is found to promote androgen-independent progression. This study was conducted to assess the mechnism of promotion of androgen-independent progression in PC-1 gene. The c-myc gene expression was tested by RT-PCR and Western blotting analyses in the LNCaP-pc-1 and LNCaP-zero cell line. After separation of cytoplasm and nulear proteins of the LNCaP-pc-1 and LNCaP-zero cell line, the beta-catenin protein was detected by Western blotting. C4-2 cell line was used to examine the effects of 10058-F4 on the PC-1 gene expression. The results of RT-PCR and Western blotting indicated that PC-1 enhanced c-myc gene expression in prostate cancer cells, PC-1 was also found to enhance beta-catenin expression in nuclear. Furthermore, a small-molecule c-Myc inhibitor, 10058-F4 represses PC-1 gene expression in C4-2 cell line. Our findings suggest that PC-1 enhances c-myc gene expression in prostate cancer cells through the Wnt/beta-catenin pathway. Meanwhile, c-myc plays a feed-forward role in enhancing PC-1 driven c-myc gene expression, and promotes prostate an-drogen-independent progression. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
