Rapid amplification and fixation of new restriction sites in the ribosomal dna repeats in the derivatives of a cross between maize andTripsacum dactyloides

Autor:	J. R. Harlan, Tuan‐Hua David Ho, Liang‐Shiou Lin
Rok vydání:	1985
Předmět:	Genetics Cell Biology Biology biology.organism_classification Molecular biology chemistry.chemical_compound Restriction site chemistry Backcrossing Tripsacum dactyloides Repeated sequence Gene Tripsacum Ribosomal DNA DNA Developmental Biology
Zdroj:	Developmental Genetics. 6:101-112
ISSN:	1520-6408 0192-253X
Popis:	Some of the derivatives of a cross of maize (Zea mays L.) × Tripsacum dactyloides (L) L (2n = 72) have abnormal development leading to strange and striking morphologies. The Tripsacum chromosomes in these “tripsacoid” maize plants (with Tripsacum-like characteristics) were eliminated and the maize chromosomes were recovered through repeated backcrossing to maize. As an initial attempt to analyze the DNA alterations in tripsacoid maize, we have detected a few restriction site changes in the ribosomal DNA repeat of these plants (Hpa II, Bal I, Sst I, Mbo II, and Sph I) and a new Sph I site was mapped to the spacer region between the 26S and 17S genes. Several possible mechanisms for the generation of a new restriction site are discussed, and we propose that the transient presence of Tripsacum genome during the backcrossing in some way induced a rapid amplification and fixation of new restriction sites in a relatively short period of time.
Databáze:	OpenAIRE
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