| Autor: |
Casaz, Paul, Boucher, Elisabeth N., Wollacott, Rachel, Ozturk, Sadettin S., Thomas Jr., William D., Wang, Yan |
| Rok vydání: |
2014 |
| DOI: |
10.13028/s238-t268 |
| Popis: |
A common challenge encountered during development of high concentration monoclonal antibody formulations is preventing self-association. Depending on the antibody and its formulation, self-association can be seen as aggregation, precipitation, opalescence or phase separation. Here we report on an unusual manifestation of self-association, formation of a semi-solid gel or “gelation”. Therapeutic monoclonal antibody C4 was isolated from human B cells based on its strong potency in neutralizing bacterial toxin in animal models. The purified antibody possessed the unusual property of forming a firm, opaque white gel when it was formulated at concentrations >40 mg/mL and the temperature was |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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