| Autor: | Dubravka Drabek, Ton de Wit, Frank Grosveld |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Small interfering RNA
RNA-induced transcriptional silencing viruses fungi RNA Biology Molecular biology chemistry.chemical_compound RNA silencing chemistry RNA interference DNA-directed RNA interference RNA polymerase Genetics Gene silencing Animal Science and Zoology Agronomy and Crop Science Biotechnology |
| Zdroj: | Transgenic Research. 11:305-310 |
| ISSN: | 0962-8819 |
| Popis: | Double-stranded RNA (dsRNA) has been shown to interfere with the function of specific genes in various invertebrate species. The application of dsRNA interference (RNAi) in vertebrates (zebrafish and mouse) is still limited to embryos and it is not clear whether the method is generally applicable. Using a transgenic mouse model we investigated whether a stably inherited dsRNA introduced as a transgene can interfere with the expression of a specific target gene in erythroid tissue during development. In our globin gene system we do not observe any specific RNA interference. We, therefore, also introduced another gene that may be involved in a mechanism of post transcriptional gene silencing (PTGS), namely RNA-dependent RNA polymerase (RdRP) that was proposed to be involved in producing RNAs that trigger PTGS in plants. However, even though the tomato RdRP is catalytically active in erythroid tissue, no RNAi was observed. |
| Databáze: | OpenAIRE |
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