| Autor: |
H. Kubinski, Priscilla Gibbs, Charles B. Kasper |
| Rok vydání: |
1972 |
| Předmět: |
|
| Zdroj: |
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 281:244-252 |
| ISSN: |
0005-2787 |
| DOI: |
10.1016/0005-2787(72)90176-1 |
| Popis: |
Purified membranes from animal cells band in CsCl density gradients at a density of about 1.2 g/cm 3 . Certain natural and synthetic nucleic acids interact with nuclear envelope and endoplasmic reticulum from rat liver to form complexes which are recovered from the gradient at the density characteristic for the membrane. Binding of nucleic acids to these membranes is remarkably specific. For example, microsomal membrane interacts only with DNA and with the co-polymer of poly(C). poly(G) while the nuclear envelope binds several natural and synthetic nucleic acids. Addition of certain non-membrane proteins during the incubation period may modify both the density of the membrane and the specificity of a particular nucleic acid-membrane interaction. Similar membrane-nucleic acid complexes were not formed with erythrocyte “ghosts” from man, dog, pig, mouse and rat. Possible biological significance of these phenomena is discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
