Formulation of Para-coumaric Acid Loaded Chitosan Nanoparticles: In Vitro Neuroprotective effect against Neuroblastoma Cell Line
| Autor: | K Krishnakumar, Dineshkumar B, Smitha K Nair, R. Rajeshkumar, Dony Lonappan |
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| Rok vydání: | 2021 |
| Předmět: |
Chemistry
medicine.drug_class Nanoparticle 02 engineering and technology 021001 nanoscience & nanotechnology Antimicrobial Coumaric acid Neuroprotection Anti-inflammatory In vitro Chitosan 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Biochemistry 030220 oncology & carcinogenesis medicine Pharmacology (medical) 0210 nano-technology Neuroblastoma cell line Pharmacology Toxicology and Pharmaceutics (miscellaneous) |
| Zdroj: | Research Journal of Pharmacy and Technology. :2286-2290 |
| ISSN: | 0974-360X 0974-3618 |
| DOI: | 10.52711/0974-360x.2021.00404 |
| Popis: | The prevalence of neurological diseases is increasing too rapidly in ageing population in world. In traditional practice of medicine of natural products has been reported to treat neurological disorders. Para-coumaric acid is a hydroxycinnamic acid and reported for various pharmacological activities such as anti-oxidant, anti-microbial, anti-cancer, anti-ulcer and anti-inflammatory effects. But clinical application of para-coumaric acid is limited due to its poor dissolution rate. Polymeric nanoparticles have been reported for targeted drug delivery system. With this information, our study focused to formulate para-coumaric acid loaded chitosan nanoparticles in order to evaluate its neuroprotective potential against neuroblastoma (SH-SY5Y) cell line. Different formulations (F1, F2, F3) of Para-coumaric acid loaded chitosan nanoparticles (PCA-NPs) was prepared by simple ionic cross-linking method. Formulated PCA-NPs were subjected to characterization methods such as SEM analysis, Drug loading and Entrapment efficiency, in-vitro drug release and Stability studies. The in vitro cyto-toxicity assay of formulated PCA-NPs was performed against neuroblastoma (SH-SY5Y) cell line. This study indicated that F2-formulationof PCA-NPs showed improved dissolution rate of drug and considerable neuro-protective activity against neuroblastoma (SH-SY5Y) cell line in a dose-dependent manner. Thus, the study concluded that nanoparticle formulation could be suitable for targeted drug delivery system against neurological disorder. |
| Databáze: | OpenAIRE |
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