Esterolytic method for determination of heparin in plasma

Autor:	G F Kapke, R D Feld, W G Owen, David L. Witte
Rok vydání:	1981
Předmět:	Chromatography Correlation coefficient medicine.diagnostic_test Chemistry Biochemistry (medical) Clinical Biochemistry Antithrombin Plasma Heparin Thrombin medicine Thromboplastin Heparin assay medicine.drug Partial thromboplastin time
Zdroj:	Clinical Chemistry. 27:526-529
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/27.4.526
Popis:	This assay for heparin is based on the heparin-accelerated rate of alpha-thrombin III. The rate or product formation from the residual active thrombin is inversely proportional to plasma heparin content. The assay can be performed manually, but our results were obtained with a discrete analyzer, the ABA-100. The assay is insensitive to concentrations of antithrombin III in plasma. Precision studies gave CVs of less than 10%. This assay was compared to the Dade Protopath heparin assay and a correlation coefficient of 0.90 was obtained (n = 62). The correlation between activated partial thromboplastin times and heparin concentrations (r = 0.67) was calculated frm results on 78 plasma specimens from 10 patients.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::80b58609fecfe89f6537b6e74d614702 https://doi.org/10.1093/clinchem/27.4.526 Zobrazit plný text záznamu