Mechanistic divergence between (4S,7R)-germacra-(1(10)E,5E)-dien-11-ol synthases from Dictyostelium purpureum and Streptomyces coelicolor

Autor:	Houchao Xu, Xinlu Chen, Feng Chen, Tobias G. Köllner, Jan Rinkel, Jeroen S. Dickschat
Rok vydání:	2021
Předmět:	Dictyostelium purpureum biology Stereochemistry Chemistry Convergent evolution Organic Chemistry Streptomyces coelicolor Physical and Theoretical Chemistry Geosmin synthase biology.organism_classification Biochemistry Sequence identity
Zdroj:	Organic & Biomolecular Chemistry. 19:370-374
ISSN:	1477-0539 1477-0520
DOI:	10.1039/d0ob02361b
Popis:	The main product of DpTPS9 from the social amoeba Dictyostelium purpureum was identified as (4S,7R)-germacra-(1(10)E,5E)-dien-11-ol that is also known as an intermediate of bacterial geosmin synthase, but the experimentally verified cyclisation mechanisms differ. Together with the low sequence identity this points to convergent evolution. The functionality of selected residues in DpTPS9 was investigated via site-directed mutagenesis experiments.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::80b00b0bfc55bd4fe95f20d05fac4888 https://doi.org/10.1039/d0ob02361b