Development of a novel enzyme-CdS nanoparticle hybrid system for the oxidation of NADH to NAD+
| Autor: | Yuki Nishibayashi, Kotaro Kobayashi, Nobuhiro Aburai, Katsuya Abe, Tomoya Horiuchi |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Polyethylenimine
biology Chemistry Mechanical Engineering education Inorganic chemistry technology industry and agriculture NADH dehydrogenase Nanoparticle Condensed Matter Physics Photochemistry Redox Catalysis chemistry.chemical_compound Mechanics of Materials Diaphorase biology.protein General Materials Science Microemulsion NAD+ kinase health care economics and organizations |
| Zdroj: | Materials Letters. 134:20-23 |
| ISSN: | 0167-577X |
| DOI: | 10.1016/j.matlet.2014.07.051 |
| Popis: | Inorganic semiconductor CdS nanoparticles (NPs) incorporating NADH dehydrogenase (diaphorase: DA) were successfully synthesized using a water-in-oil microemulsion method. The X-ray diffraction pattern of the enzyme-CdS NPs was assigned to a cubic structure CdS phase. The catalytic effect of enzyme-CdS NPs hybrid systems on the oxidation of dihydronicotinamide adenine dinucleotide (NADH) to nicotinamid adenine dinucleotide (NAD + ) was investigated. Enzyme-CdS NPs incorporating DA and ferrocene-modified polyethylenimine (Fc-PEI) (1:0.2, w/w) exhibited a significantly increased rate of NADH oxidation under illumination compared with pure CdS NPs. The redox mediator Fc-PEI acted to transfer electrons between the valence band of the CdS NPs and the flavin-bound enzyme during the oxidation reaction. Switchable oxidation of NADH in a colloidal solution of the enzyme-CdS NPs was induced with respect to the dark/light cycle. The catalytic properties of the present CdS NPs activated with an enzyme towards NADH oxidation might have future importance in biochemistry and medical applications. |
| Databáze: | OpenAIRE |
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