Lymphokine-activated killer (LAK) cells

Autor: Zuhair K. Ballas, Wendy L. Rasmussen
Rok vydání: 1991
Předmět:
Zdroj: Cellular Immunology. 134:296-313
ISSN: 0008-8749
DOI: 10.1016/0008-8749(91)90304-t
Popis: Normal murine splenocytes cultured with IL2 for 6, but not 3, days contained an NK1.1 + , CD3 + lytically active subset. These lymphocytes were not derived from NK1.1 + precursors since NK1.1 + cells, purified by flow cytometry, failed to express CD3, as determined by the 145-2C11 mAb, on their surface even after culture with IL2 for 6 days. Instead, the precursors of the NKl.1 + , CD3 + effectors were contained in a B cell-depleted CD4 − , CD8 − , NK1.1 − splenic subset. Freshly obtained CD4 − , CD8 − , NK1.1 − splenocytes were mostly CD3 + , CD5 + , B220 − , had no spontaneous lytic activity against YAC-1, and were unable to mediate anti-CD3 directed lysis against FcR-bearing target cells. Culture of the CD4 − , CD8 − , NK1.1 − splenocytes with IL2, for 6 days, resulted in the development of NK1.1 + , CD3 + , B220 + effectors 40% of which were CD5 dim and 20–25% of which expressed TCR-β8 as determined by the F23.1 mAb. The acquisition of NK1.1, B220, and lytic activity by this triple-negative subset was readily inhibited by cyclosporine A (CSA). On the other hand, CSA had no effect on the acquisition of B220 or lytic activity by NK1.1 + precursors obtained by flow cytometry sorting. Moreover, all of the NK1.1 + cells generated by IL2 culture of splenocytes obtained from mice depleted of NK1.1 + lymphocytes (by in vivo injection of anti-NK1.1 mAb) coexpressed CD3 on their surface and were thus distinct from classical NK cells. These findings demonstrate tjiat splenic NK cells do not express or acquire CD3; that the NK1.1 + , CD3 + LAK effectors are derived from an NK1.1 − precursor; and that CSA is exquisitely selective in its inhibitory effect on LAK generation.
Databáze: OpenAIRE
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