In vitro measures of membrane changes reveal differences between red blood cells stored in saline-adenine-glucose-mannitol and AS-1 additive solutions: a paired study
| Autor: | Geraldine Healey, Amrita Sran, Philip J. Norris, Rosemary L. Sparrow, Margaret F. Veale |
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| Rok vydání: | 2013 |
| Předmět: |
biology
medicine.diagnostic_test Immunology hemic and immune systems Hematology Phosphatidylserine medicine.disease Hemolysis In vitro Flow cytometry Andrology chemistry.chemical_compound Membrane chemistry Biochemistry hemic and lymphatic diseases medicine biology.protein Immunology and Allergy Glycophorin Mannitol circulatory and respiratory physiology medicine.drug Whole blood |
| Zdroj: | Transfusion. 54:560-568 |
| ISSN: | 0041-1132 |
| Popis: | Background Saline-adenine-glucose-mannitol (SAGM) and a variant solution, AS-1, have been used for more than 30 years to preserve red blood cells (RBCs). Reputedly these RBC components have similar quality, although no paired study has been reported. To determine whether differences exist, a paired study of SAGM RBCs and AS-1 RBCs was conducted to identify membrane changes, including microparticle (MP) quantitation and in vitro RBC–endothelial cell (EC) interaction. Study Design and Methods Two whole blood packs were pooled and split and RBCs were prepared (n = 6 pairs). One pack was suspended in SAGM and one in AS-1. Samples were collected during 42 days of refrigerated storage. RBC shape and size and glycophorin A (GPA)+ and phosphatidylserine (PS)+ MPs were measured by flow cytometry. RBC adhesion to ECs was determined by an in vitro flow perfusion assay. Routine variables (pH, hemolysis) were also measured. Results Compared to SAGM RBCs, AS-1 RBCs had lower hemolysis (p |
| Databáze: | OpenAIRE |
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